A novel microcurrent dressing for wound healing in a rat skin defect model

Chao Yu; Zhi-Xiu Xu; Yan-Hui Hao; Ya-Bing Gao; Bin-Wei Yao; Jing Zhang; Bing Wang; Zong-Qian Hu; Rui-Yun Peng

doi:10.1186/s40779-019-0213-x

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A novel microcurrent dressing for wound healing in a rat skin defect model

RESEARCH | Updated：2023-06-05

- A novel microcurrent dressing for wound healing in a rat skin defect model
- A novel microcurrent dressing for wound healing in a rat skin defect model
- MMR 2020年7卷第2期页码：190-198
- Affiliations：
  
  1.Department of Experimental Pathology, Beijing Institute of Radiation Medicine, Beijing 100850, China
  2.Department of Internal Neurology, The First Affiliated Hospital/Xinxiang Medical University, Xinxiang 453100, Henan, China
  3.Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100850, China
- Author bio：
  
  * huzongqian@gmail.com;
  ruiyunpeng18@126.com
- Funds：
  
  the National Natural Science Foundation of China(61402486)
- DOI：10.1186/s40779-019-0213-x
  中图分类号：
- 纸质出版：2020-06
- Accepted：
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A novel microcurrent dressing for wound healing in a rat skin defect model[J]. MMR, 2020,7(2):190-198.

Cite this article as: Yu et al.: A novel microcurrent dressing for wound healing in a rat skin defect model. Mil Med Res, 2019, 6: 22
A novel microcurrent dressing for wound healing in a rat skin defect model[J]. MMR, 2020,7(2):190-198. DOI： 10.1186/s40779-019-0213-x.

Cite this article as: Yu et al.: A novel microcurrent dressing for wound healing in a rat skin defect model. Mil Med Res, 2019, 6: 22 DOI： 10.1186/s40779-019-0213-x.

摘要

Abstract

Background:

The exogenous application of low-intensity electric stimulation (ES) may mimic a natural endogenous bioelectric current and accelerate the repair process of skin wounds. This study designed a novel microcurrent dressing (MCD) and evaluated its potential effects on wound healing in a rat skin defect model.

Methods:

First

wireless ES was integrated into a medical cotton cushion to fabricate the MCD

and its electrical property was examined by using a universal power meter. Then

animal experiments were conducted to evaluate the MCD’s effect. Forty-five rats were randomized into control (Con) group

Vaseline gauze (VG) group and MCD group. A full-thickness round skin incision 1.5 cm in diameter was made on the back of each animal. Apart from routine disinfection

the Con rats were untreated

whereas the other two groups were treated with VG or MCD. On days 3

7 and 14 post injury

the wound areas were observed and measured using image analysis software following photography

and the skin samples were harvested from wound tissue. Then

histopathological morphology was observed routinely by hematoxylin and eosin (HE) staining; tumor necrosis factor α (TNF-α) and interleukin (IL)-1β expression were detected by Western blotting. Vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF) expression were detected with immunohistochemistry.

Results:

The MCD generated a sf electric potential greater than 0.95 V. Animal experiments showed that the woundhealing rate in the MCD group was significantly increased compared with the Con and VG groups (

0.05 or

0.01). Histopathological observation revealed an alleviated inflammatory response

induced vascular proliferation and accelerated epithelization in the MCD group. Moreover

samples from the MCD group expressed reduced TNF-α and IL-1β levels and increased VEGF and EGF levels compared with those of the other two groups (

0.05 or

0.01). However

no significant difference was noted between the Con and VG groups at each time point.

Conclusions:

The MCD generates a stable and lasting ES and significantly promotes wound healing by reducing inflammation duration and increasing growth factors expression. Thus

MCD may act as a promising biomaterial device for skin wound healing.

关键词

Keywords

references

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