Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity
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Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity
Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity
解放军医学杂志(英文版)1986年第2期 页码:159-164
Affiliations:
1. Department of Gastroenterology The First Affiliated Hospital
2. The Fourth Military Medical College
3. Xian
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纸质出版:1986
Accepted:
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Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J]. 解放军医学杂志(英文版), 1986,(2):159-164.
[1]张忠兵,张学庸,陈希陶.Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J].Journal of Medical Colleges of PLA,1986(02):159-164.
Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J]. 解放军医学杂志(英文版), 1986,(2):159-164.DOI:
[1]张忠兵,张学庸,陈希陶.Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J].Journal of Medical Colleges of PLA,1986(02):159-164.DOI:
Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity
摘要
Abstract
<正> Acid α-naphthyl acetate esterase(ANAE)activity was used todemonstrate T lymphocytes in paraffin-embedded gastrointestinal tissuesand perigastric lymph nodes
one or more reddish-brown dots could berecognized in the cytoplasm of T lymphocytes
diffuse reddish-brownANAE products were also present in the cytoplasm of macrophages
epithelial and glandular cells.B lymphocytes were ANAE negative.Itwas demonstrated that ANAE cytochemical staining technique could beused to differentiate T lymphocytes.This method is simple and easy tocarry out in most hospitals.The percentage of T lymphocytes in essentially normal gastric mucosawas about 9.8% There was no significant difference in fundi andantra.The intraepithelial cells were located at the bottom of epithe-lial cells.In lamina propria T lymphocytes were distributed near the ba-sal layer of the epithelium.This might be related to the perception ofantigenic stimulation and regulation of immunologic reaction.
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