Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA

张世忠; 李良寿; 欧阳修; 李恩善; 黄其全; 汪美先

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Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA

Updated：2026-03-12

- Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA
- Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA
- 解放军医学杂志（英文版） 1986年第3期页码：246-252
- Affiliations：
  
  1. Department of Epidemiology Fourth Military Medical College
  2. ,Xi’an
  3. Department of Microbiology
  4. Fourth Military Medieal College
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1986
- Accepted：
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Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA[J]. 解放军医学杂志（英文版）, 1986,(3):246-252.

[1]张世忠,李良寿,欧阳修,李恩善,黄其全,汪美先.Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA[J].Journal of Medical Colleges of PLA,1986(03):246-252.
Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA[J]. 解放军医学杂志（英文版）, 1986,(3):246-252. DOI：

[1]张世忠,李良寿,欧阳修,李恩善,黄其全,汪美先.Use of Monoclonal Antibodies in Detection of Clostridium botulinum Type B Toxin by ELISA[J].Journal of Medical Colleges of PLA,1986(03):246-252. DOI：

摘要

Abstract

<正> A sensitive and specific double-sandwich ELISA system utili-zing ascites fluid containing monoclonal antibody as a coatingantibody was developed for the detection of Clostridium botulinumtoxoid

toxin and artificially contaminated food specimens.Theminimal detectable amount（MDA）of toxoid varied from 4 to 31ng/ml.The lowest concentration for cross-reaction to botulinum AToxoid ranged from 4 to 8μg/ml.The specific index obtained wasbetween 250 and 1000.The MDA of botulin B was 16 ng/ml（4 LD50/ml）

whereas that of type A toxin was larger than 32 μg/ml（301LD50/ml）;the specific index was over 2000.The MDA of botulin Bin artificially contaminated food varied from 0.67 to 7.8 LD50/mland no cross-reaction was shown between types A and B.The ELISAsystem is close to RPHA in sensitivitv but is apparently superiorto the latter in specificity.

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