Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum
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Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum
Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum
解放军医学杂志(英文版)1988年第2期 页码:110-115
Affiliations:
1. Department of Malaria Immunology First Medical College of PLA
2. ,Guangzhou
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纸质出版:1988
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Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J]. 解放军医学杂志(英文版), 1988,(2):110-115.
[1]王旗,李英杰.Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J].Journal of Medical Colleges of PLA,1988(02):110-115.
Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J]. 解放军医学杂志(英文版), 1988,(2):110-115.DOI:
[1]王旗,李英杰.Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum[J].Journal of Medical Colleges of PLA,1988(02):110-115.DOI:
Purification and Analysis of Soluble Antigens in Short Term Serum-Free in vitro Culture Medium of Plasmodium Falciparum
摘要
Abstract
<正> Supernatants collected from the short term in vitro serum-free cultures ofPlasmodium falciparum (Pf) schizont-infected erythrocytes were detected and analysed bycounterimmuno-electrophoresis (CIE) and enzyme-linked immunoabsorbent assay (ELISA).Soluble antigens were purified from the supernatants by immunoabsorbent chroma-tography. It was found that soluble antigens similar to the sonicated Pf antigens inimmunospecificity were present in the supernatants and no erythrocyte membrane antigenswere detectable in the affinity-purified supernatant antigens (Ps-Ag).
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