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Previous work in our laboratory showed that

after scalding

there wasnot only a decrease of rat liver cytosol [

3

H]-Dexamethasone specific binding sitesbut also an increase of apparent dissociation constant of [

3

H]-Dex and change ofsedimentation coefficient of glucocorticoid receptor.The present study was carriedout using photoaffinity labeling of glucocorticoid receptor in rat liver cytosol.Itwas found that there was no significant difference between molecular weights ofglucocorticoid receptor of normal and scalded rats determined by SDS-polyacrylamide gel electrophoresis.Our result suggests that chnage ofsedimentation coefficient is not due to alteration of molecular weight ofglucocorticoid receptor binding subunit.