Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization

冯根生; 叶天星; 杨嗣坤

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Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization

Updated：2026-03-12

- Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization
- Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization
- 解放军医学杂志（英文版） 1986年第3期页码：232-238
- Affiliations：
  
  1. Department of Microbiology and Laboratory of Molecular Genetics Second Military Medical College
  2. ,Shanghai
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1986
- Accepted：
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Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization[J]. 解放军医学杂志（英文版）, 1986,(3):232-238.

[1]冯根生,叶天星,杨嗣坤.Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization[J].Journal of Medical Colleges of PLA,1986(03):232-238.
Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization[J]. 解放军医学杂志（英文版）, 1986,(3):232-238. DOI：

[1]冯根生,叶天星,杨嗣坤.Interferon-gamma Induced by Pokeweed Mitogen or Esculentoside and Its Partial Purification and Characterization[J].Journal of Medical Colleges of PLA,1986(03):232-238. DOI：

摘要

Abstract

正

Interferon-gamma（IFN-γ）was induced by pokeweed mitogen（PWM）or esculentoside in human splenic lymphocytes.PWM-inducedIFN-γ was partially purified to a specific activity of 10

RU/mgprotein by batch adsorption on controlled-pore glass（CPG）beadsand desorption by ethylene glycol in phosphate-buffered saline（PBS）.Use of Chinese made micro-pore glass（MPG）instead of CPG topurify IFN-γ showed similar purification efficacy.Both CPG andMPG recovered could be regenerated for repeated use.The yieldfrom glass beads was further purified to a specific activity of 10

RU/mg protein

with about 78% recovery

by the anti-new-borncalf serum antibodies-Sepharose 4B（ACS）column chromatography.Almost all the antiviral activity of the IFN-γ induced by PWM oresculentoside was destroyed by adjusting to pH 2 or heating to 56℃

and was neutralized by monoclonal antibody to human interferon-γ（Hu IFN-γ）

thereby the product was identified as Hu IFN-γ.Bio-gel P-200 chromatography resulted in two peaks of activity for PWM-induced IFN

a major one with molecular weight（MW）of 40

000-45

700 and a minor one of 25

700

but only one peak of 45

700-49

000for esculentoside-induced IFN.

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