Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties

刘明耀; 张亚霏

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Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties

Updated：2026-03-12

- Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties
- Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties
- 解放军医学杂志（英文版） 1987年第1期页码：83-87
- Affiliations：
  
  1. Department of Pathophysiology Second Military Medical College
  2. ,Shanghai
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1987
- Accepted：
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Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties[J]. 解放军医学杂志（英文版）, 1987,(1):83-87.

[1]刘明耀,张亚霏.Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties[J].Journal of Medical Colleges of PLA,1987(01):83-87.
Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties[J]. 解放军医学杂志（英文版）, 1987,(1):83-87. DOI：

[1]刘明耀,张亚霏.Isolation of Bovine Pulmonary Surfactant and Analysis of Its Chemical Composition and Physicochemical Properties[J].Journal of Medical Colleges of PLA,1987(01):83-87. DOI：

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Abstract

正

Bovine Pulmonary surfaetant was obtained by endotracheal lavage oflungs from newly slaughtered calves fallowed by differential centrifugation.Lipidextracts of bovine surfactant contained 86.1±2.1% of phospholipids（（?）±SD）

1.8

1.1% of protein

and smaller amounts of cholesterol

fatty acids

glyceridesand carbohydrate.Phosphatidylcholine（83.4±5.6%）accounted for most of thephospholipids

of which disaturated pbosphatidylcholine was 76.0±4.7%.Thephysic（?）chemical properties of bovine sarfactant were measured with a modifiedWilhelmy film balance made by the authors.The minimum surface tension was2.4±1.5mN/m

stability index 1.78±0.15.surface eompressibility at 10mN/m0.022±0.004m/mN

and surface concentration of phospholipids at 10 mN/m1.112±0.283 nmol/cm

.The surfactant spread rapidly and could be quicklyadsorbed to the air liquid interface.The results show that the bovine pulmonarysurfactant we isolated is able to meet the criteria for investigations of surfactantreplacement therapy.

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