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The performances of the isolated working rat hearts perfusedwith modified K-H solution were stable for 90 min of perfusion time.However

the contractility and the total cardiac output decreasedsignificantly after 120 min. The acute regional ischemia produced by theligation of left main coronary artery in the isolated working rat hearts wasfollowed by decrease in cardial power production and increase in creatinephosphokinase release. Coronary flow restored to the preligation valueimmediately after the beginning of reperfusion following 30 min regionalischemia

but the cardiac function （as measured by total cardiac output andpower production） did not recover significantly and the creatinephosphokinase release even further increased during 15 min reperfusion.1.4 × 10

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M puerarin significantly increased coronary flow and did notaffect other mechanical performances of the normal isolated working rathearts. However

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M propranolol as well as 1.4 × 10

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M puerarinreduced significantly the creatine phosphokinase release from the regionalischemic-reperfused myocardium and accelerated the recovery of the cardiacfunction during reperfusion time in the isolated working rat hearts. Theresults suggested that both puerarin and propranolol have direct benificialeffects on the ischemic-reperfused myocardium.