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A quantitative method using fluorescence spectro-photometer to detect theinterleukin 2 reocptor （IL 2R） on the surface of mononuclear cells in normal humanperipheral blood is described. For expression of IL 2R

the optimum conoentration of PHA andCon A were 100 μg/ml and 10 μg/ml respectively. The PHA induced effect on mononuclear cellswas better than Con A induced one. The peak value of IL 2R cxpression was at the 24th h afterstimulation The optimum dilution of the first antibody （anti-Tac） was 1: 50

while the dilution ofthe second antibody（fluorescein conjugated rabbit anti-mousc IgG） was 1: 32 Their optimumincubation period was 20 and 10 min repectively. The concentration of fluorcscein-conjugatedantibody per 1×10

6

mononuclear cells from the same normal human peripheral blood was5.22±0.45×10

-10

mol

and the coefficient of variation was 8. 6%. The concentration offluorescein-conjugated antibody per 1×10

6

PBMC from five healthy individuals was 4. 8±0. 97×10

-10

mol Therefore this assay system is stable and quite reproducible.