Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases

李奇芬; 朱纯吾; 张士敏; 王慧芬

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Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases

Updated：2026-03-12

- Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases
- Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases
- 解放军医学杂志（英文版） 1988年第2期页码：100-104
- Affiliations：
  
  1. Department of Infectious Diseases Southwest Hospital
  2. Third Military College
  3. ,Chongqing
  4. Department of Clinical Pathology
  5. No. Hospital of PLA,302
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1988
- Accepted：
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Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases[J]. 解放军医学杂志（英文版）, 1988,(2):100-104.

[1]李奇芬,朱纯吾,张士敏,王慧芬.Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases[J].Journal of Medical Colleges of PLA,1988(02):100-104+201.
Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases[J]. 解放军医学杂志（英文版）, 1988,(2):100-104. DOI：

[1]李奇芬,朱纯吾,张士敏,王慧芬.Preliminary Studies of in situ Hybridization in Routine Paraffin Embedded Liver Tissue Sections with iotinylated HBV DNA Probe:Localization and Morphology of HBV DNA in Various Types of Liver Diseases[J].Journal of Medical Colleges of PLA,1988(02):100-104+201. DOI：

摘要

Abstract

<正> The in situ hybridization of HBV DNA with biotinylated probe informalin-fixed and paraffin-embedded liver tissue sections was performed and thelocalization and morphology of HBV DNA in various types of chronic liver diseases wereobserved.25 out of the 75 cases studied were HBV DNA positive （33.3%）.HBV DNA inhepatocytes was demonstrated as numerous coarse grains in cytoplasm and occasionally afew grains in individual nuclei.The distribution of the HBV DNA positive hepatocytes inthe sections was in 3 types

i.e.

the diffuse type （Type I

4/25

16%）

the focal type （Type Ⅱ

20/25

80%）

and the scattered type （Type Ⅲ

1/25

4%）.The HBV DNA grains incytoplasm might be condensed （Type a） or sparsed （Type b） depending on the number of theHBV DNA grains.The different distribution patterns and the amount of HBV DNAgrains might be a reflection of the replication activity and the state of infectivity of HBV.HBV DNA positive hepatocytes were found only in the tissues contiguous to the cancertissue in cases with hepatocellular carcinoma with cirrhosis.

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