Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum

李纪良; 巢穗; 欧阳明辉; 李英杰

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Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum

Updated：2026-03-12

- Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum
- Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum
- 解放军医学杂志（英文版） 1989年第1期页码：57-59
- Affiliations：
  
  Department of Malaria Immunology First Military Medical University Guangzhou
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- 纸质出版：1989
- Accepted：
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Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum[J]. 解放军医学杂志（英文版）, 1989,(1):57-59.

[1]李纪良,巢穗,欧阳明辉,李英杰.Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum[J].Journal of Medical Colleges of PLA,1989(01):57-59.
Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum[J]. 解放军医学杂志（英文版）, 1989,(1):57-59. DOI：

[1]李纪良,巢穗,欧阳明辉,李英杰.Preparation and Identification of Rat Monoclonal Anti-idiotope Antibody to the Antibody against Erythrocytic Stages of Plasmodium Falciparum[J].Journal of Medical Colleges of PLA,1989(01):57-59. DOI：

摘要

Abstract

<正> A hybridoma cell line secreting monoclonal antibody to idiotope of the monoclonalantibody 94D1 specific for asexual erythrocytic stages of Plasmodium falciparum was established byfusion of SP2/0 mouse myeloma cells with spleen cells of Wistar rats immunized with monoclonalIgG of 94D1 purified from ascitic fluid of BALB/C mouse by affinity chromatography on ProteinA- Sepharose CL- 4B. Specificity of the anti - idiotope antibody （anti - Id）

41RF5

was deter-mined with indirect enzyme-linked immunosorbent assay （ELISA）. The results showedthat 41RF5 reacted only with 94D1 IgG2b

not with normal mouse IgG and other seven mousemonoclonal antibodies - five specific for erythrocytic stages of P. falciparum

one for erythrocyticstages of P. inui

and one for Dengue fever virus type III

which indicates that 41RF5 does recognizespecifically the idiotope of 94D1 monoclonal antibody. In 41RF5 heterohybrid culture supernatant

anti-Id titre measured by ELISA was above 1:1 280. Up to the present

the heterohybrid cell linehas been cultured stably for 16 months.

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