Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas
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Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas
Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas
解放军医学杂志(英文版)1989年第3期 页码:187-190
Affiliations:
1. Department of Biochemistry Nanfang Hospital First Military Medical University Guangzhou
3. Department of Obstetrics and Gynecology Nanfang Hospital First Military Medical University Guangzhou
Author bio:
Funds:
National Natural Science Foundation of China
DOI:
中图分类号:
纸质出版:1989
Accepted:
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Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas[J]. 解放军医学杂志(英文版), 1989,(3):187-190.
[1]高基民,徐钤,陈碧魂.Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas[J].Journal of Medical Colleges of PLA,1989(03):187-190.
Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas[J]. 解放军医学杂志(英文版), 1989,(3):187-190.DOI:
[1]高基民,徐钤,陈碧魂.Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas[J].Journal of Medical Colleges of PLA,1989(03):187-190.DOI:
Detection of Human Papillomavirus Type 16 DNA in Cervical Carcinomas
摘要
Abstract
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Accurate typing of the different human papillomavirus types is csscntial in view of the differ-ent pathological potential of the common virus types of human papillomavirus (HPV) present in thecervix. We have developed hybridization
washing and autoradiography conditions that minimize thecross-hybridization among different specific types of HPV so as to allow clear - cut type assignmentthrough practical dot blot hybridization technique using nylon membrane and
35
S - labeled HPV - 16DNA probe. Under these conditions seventeen of thirty (56.7%) of squamous cell carcinomas of thecervix uteri obtained from Tianjin women were detected in the presence of HPV - 16 DNA.
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