In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line

黎松; 谈立松; 张学庸; 陈希陶; 樊代明; 潘惠忠; 胡继旺

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In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line

Updated：2026-03-12

- In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line
- In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line
- 解放军医学杂志（英文版） 1989年第3期页码：217-220
- Affiliations：
  
  1. Laboratory of Gastroenterology Xijing Hospital Fourth Military Medical University Xi’an
  2. Department of Biochemistry Shanghai Institute of Lung Tumors Shanghai
  3. Laboratory of Gastroenterology Xijing Hospital Fourth Military Medical University Xi’an
- Author bio：
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- 纸质出版：1989
- Accepted：
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In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line[J]. 解放军医学杂志（英文版）, 1989,(3):217-220.

[1]黎松,谈立松,张学庸,陈希陶,樊代明,潘惠忠,胡继旺.In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line[J].Journal of Medical Colleges of PLA,1989(03):217-220.
In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line[J]. 解放军医学杂志（英文版）, 1989,(3):217-220. DOI：

[1]黎松,谈立松,张学庸,陈希陶,樊代明,潘惠忠,胡继旺.In Vitro Cytotoxicity of Conjugate of Mitomycin C with Murine Monoclonal Antibody against Gastric Cancer Cell Line[J].Journal of Medical Colleges of PLA,1989(03):217-220. DOI：

摘要

Abstract

正

In the present study

the conjugate of mitomycin C （MMC） with a murine monoclonalantibody against gastric cancer

MG11

was prepared and its cytotoxicity on gastric cancer cell lineKATOⅢ was determined. MMC was modified with glutaric anhydride to introduce a carboxylicgroup at N-lα position. The MMC derivative was then treated with N-hydroxysuccinimide anddicyclohexylcarbodimide to from MMC active ester

which was combined with monoclonal antibodyMG11 for preparation of MG11-MMC conjugate

which showed highly selective cytotoxicity uponhuman gastric cancer cell line KATOⅢ in culture. In the 48h exposure test

the cytotoxicity ofMG11-MMC conjugate on target cells was found to be similar to that of free MMC. The conjugatehad only a little effect upon nontarget cells （human normal embryonic lung cell line SL

）. The re-sults suggest that the selective cytotoxicity on target cells of MG11-MMC is mediated by theantibody.

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