Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation
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Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation
Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation
解放军医学杂志(英文版)1990年第3期 页码:251-256
Affiliations:
1. Department of Ophthalmology Xijing Hospital
2. Fourth Military Medical University
3. ,Xi’an,710032
4. Laboratory of Electron Microscopy
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纸质出版:1990
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Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation[J]. 解放军医学杂志(英文版), 1990,(3):251-256.
[1]张廷钺,于纯智,马吉献,彭秀军,梁厚成,魏正乾,刘健,谷法有,李学荣,杨传红.Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation[J].Journal of Medical Colleges of PLA,1990(03):251-256.
Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation[J]. 解放军医学杂志(英文版), 1990,(3):251-256.DOI:
[1]张廷钺,于纯智,马吉献,彭秀军,梁厚成,魏正乾,刘健,谷法有,李学荣,杨传红.Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation[J].Journal of Medical Colleges of PLA,1990(03):251-256.DOI:
Morphological Characteristics of the Endothelial Ceils of Corneas of Rabbits Preserved by Desiccation
摘要
Abstract
<正> The endothelial cells of the corneas of rabbits preserved by desiccation for vari-ous duration showed no hexagonal form of normal cells but broad bean-like nuclci afterdouble staining with alizarin red and trypan blue.Scanning electron microscopic examina-tion revealed interdigitation at the intercellular junction together with intercellularvacuoles and cellular edema.Transmission electron microscopic examination showedincomplete nuclear membrane
karyopyknosis
vacuoles and remnant organellae in thecytoplasm.Partial penetrating keratoplasties were done in 37 rabbits(37 eyes)with pre-served corneas.The grafts remained transparent for a minimum of 4 days to a maximumof over 1 year
and 7 grafts remained transparent for more than 30 days.Staining ofthe transparent grafts revealed normal hexagonal endothelial cells in mosaic arrange-ment.We assumed that after transplantation of the preserved cornea the endothelial cellsregained their activities owing to the nutrient effect of the aqueous humor.
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