Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria

缪为民; 管惟滨; 徐晓春; 周元昌; 陆德如; 董蓓华; 陈蕊雯

Your Location：

Home >

Browse articles >

Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria

Updated：2026-03-12

- Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria
- Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria
- 解放军医学杂志（英文版） 1993年第3期页码：226-230
- Affiliations：
  
  1. Departments of Parasitology Second Military Medical University Shanghai
  2. ,200433
  3. Departments of Molecular Genetics Second Military Medical University
  4. Departments of Immunology
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1993
- Accepted：
Scan QR Code
Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria[J]. 解放军医学杂志（英文版）, 1993,(3):226-230.

[1]缪为民,管惟滨,徐晓春,周元昌,陆德如,董蓓华,陈蕊雯.Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria[J].Journal of Medical Colleges of PLA,1993(03):226-230.
Evaluation of a sulfonated DNA probe （sulfoprobe） for diagnosis of falciparum malaria[J]. 解放军医学杂志（英文版）, 1993,(3):226-230. DOI：

[1]缪为民,管惟滨,徐晓春,周元昌,陆德如,董蓓华,陈蕊雯.Evaluation of a sulfonated DNA probe (sulfoprobe) for diagnosis of falciparum malaria[J].Journal of Medical Colleges of PLA,1993(03):226-230. DOI：

摘要

Abstract

<正> In this study

the DNA probe pPF14 was nonradioactively labelled by sulfo-modifica-tion

and used in a dot blot hybridization assay for detection of P.falciparum.The resultsshowed that the sulfoprobe successfully detected 25pg purified DNA and 0.001% parasitemia ofcultured P.falciparum

and did not react with human leukocyte DNA.In the study of 179clinical blood samples of malaria patients from Yunnan province

the DNA probe results corre-lated well with blood smear ones.Of 107 P.falciparum samples determined by microscope ex-amination

99 were positive by sulfoprobe （92.5%）;Of 72 P.vivax samples

1 was crosslyreacted;no cross reactions were found with any of 48 normal blood samples.It is indicated thatsulfoprobe may be useful in specific diagnosis and epidemiological investigation of P.falciparuminfection.

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据