A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction

周荣; 梁炽森; 骆抗先

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A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction

Updated：2026-03-12

- A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction
- A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction
- 解放军医学杂志（英文版） 1992年第1期页码：17-21
- Affiliations：
  
  1. Department of Infectious Diseases Nanfang Hospital
  2. First Military Medical University
  3. ,Guangzhou,510515
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1992
- Accepted：
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A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction[J]. 解放军医学杂志（英文版）, 1992,(1):17-21.

[1]周荣,梁炽森,骆抗先.A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction[J].Journal of Medical Colleges of PLA,1992(01):17-21.
A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction[J]. 解放军医学杂志（英文版）, 1992,(1):17-21. DOI：

[1]周荣,梁炽森,骆抗先.A study on diagnosis of post—transfusion hepatitis C with polymerase chain reaction[J].Journal of Medical Colleges of PLA,1992(01):17-21. DOI：

摘要

Abstract

<正> To study the diagnosis of hepatitis C viremia

17 patients（including 14 in acute phase）with post-transfusion non-A

non-B hepatitis（NANBH）were investigated by nested polymerasechain reaction after reverse transcription with 3 sets of primers complemented to different regions ofthe viral sequence.Hepatitis C virus（HCV）RNA was detected in 6（35%）cases with reverse tran-script polymerase chain reaction（RT-PCR）using the primers according to the Japanese clones;in13（76%）using the primers according to the non-coding region which is highly conserved in theviral sequence

and in 16（94%）using both or either one of these two sets of primers.However

no HCV RNA was detected with the primers according to the Chiron prototype.The nucleotide se-quence of HCV is quite variable

and the virus level in blood is very low

therefore

in order to getprecise detection

it is suggested to do nested RT-PCR with several sets of primers complementaryto different conserved regions.

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