Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells

丁彦青; 蔡俊杰; 张进华; 李春德; 朱梅刚; 王小宁; 罗荣城

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Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells

Updated：2026-03-12

- Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells
- Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells
- 解放军医学杂志（英文版） 1993年第4期页码：356-359
- Affiliations：
  
  1. Department of Pathology
  2. Immunology
  3. Padiatrics of Nan fang Hospital First Military Medical University
  4. ,Guangzhou,510515
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1993
- Accepted：
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Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells[J]. 解放军医学杂志（英文版）, 1993,(4):356-359.

[1]丁彦青,蔡俊杰,张进华,李春德,朱梅刚,王小宁,罗荣城.Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells[J].Journal of Medical Colleges of PLA,1993(04):356-359.
Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells[J]. 解放军医学杂志（英文版）, 1993,(4):356-359. DOI：

[1]丁彦青,蔡俊杰,张进华,李春德,朱梅刚,王小宁,罗荣城.Quantitative observation by enzyme cytochemistry of human rectal adenocarcinoma cells killed by LAK cells[J].Journal of Medical Colleges of PLA,1993(04):356-359. DOI：

摘要

Abstract

<正> Activity of succinic dehydrogenase（SDH）and acid phosphatase（AcPase）of effector-target cells during the process of LAK cells killing HR8348 cells was estimated by enzyme cyto-chemistry technique.SHD positive granules and AePase gray level were assayed with MIAS-300image analyser.The results showed:（1）After cocultivation of effector and target cells for vari-ous times

the activity of AcPase of HR8348 cells was apparently higher than that of the controlgroup

and it increased following prolonged coincubation.SDH activity of target cells increasedmarkedly within 30 and 60 rain cocultivation

but became low after 90 min treatment.（2）Ac-Pase content within LAK cells at 60

120

180 and 240 rain cocultivation was significantlyhigher than that of control group（P<0.01）.The phenomenon of high AcPase and SDH activitywithin effector-target cells indicates that the function of the two types of cells was in an activestate.At the early stage of effector-target combining

the increase of SDH with HR8348 cellsmay be related to defensive function of the target cells.Higher AcPase activity of target cells in-dicates the activation of lysosomal enzyme which serves as the material basis for autolysis of thecells.

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