Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
解放军医学杂志(英文版)1993年第2期 页码:192-196
Affiliations:
1. Burn Research Institute Southwest Hospital
2. Third Military Medical College
3. ,Chongqing,630038
Author bio:
Funds:
DOI:
中图分类号:
纸质出版:1993
Accepted:
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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J]. 解放军医学杂志(英文版), 1993,(2):192-196.
[1]张敏,尤忠义,雷政宏,王风君.Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J].Journal of Medical Colleges of PLA,1993(02):192-196.
Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J]. 解放军医学杂志(英文版), 1993,(2):192-196.DOI:
[1]张敏,尤忠义,雷政宏,王风君.Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J].Journal of Medical Colleges of PLA,1993(02):192-196.DOI:
Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
摘要
Abstract
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正
>
Modification was made on the reversed-phase high performance liquidchromatography(RP-HPLC)with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B
4
(LTB
4
)and 5-hydroxyeicosatetraenoic acid(5-HETE).Itwas found that the modified method has the merits of simpler procedures
shortertesting time and more satisfactory efficacy.
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