Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction

马维芳; 宋敏; 李进; 杨海涛; 周殿元; 徐湘民; 张基增

Your Location：

Home >

Browse articles >

Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction

Updated：2026-03-12

- Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction
- Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction
- 解放军医学杂志（英文版） 1993年第4期页码：395-399
- Affiliations：
  
  1. Department of Molecular Biology
  2. Department of Histology and Embryology
  3. Department of Gastroenterology Nan fang Hospital
  4. First Military Medical University
  5. ,Guangzhou,510515
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1993
- Accepted：
Scan QR Code
Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction[J]. 解放军医学杂志（英文版）, 1993,(4):395-399.

[1]马维芳,宋敏,李进,杨海涛,周殿元,徐湘民,张基增.Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase (?)hain reaction[J].Journal of Medical Colleges of PLA,1993(04):395-399.
Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase （?）hain reaction[J]. 解放军医学杂志（英文版）, 1993,(4):395-399. DOI：

[1]马维芳,宋敏,李进,杨海涛,周殿元,徐湘民,张基增.Detection and amplification of Helicobacter pylori urease gene A in gastric biopsies by using nested polymerase (?)hain reaction[J].Journal of Medical Colleges of PLA,1993(04):395-399. DOI：

摘要

Abstract

<正> A nested polymerase chain reaction（N-PCR）for the spegific detection of Helicobacterpylori（H.pylori）was developed with two primer pairs（nested primers）derived from ureasegene A of H.pylori.The N-PCR was used to detect 21 different samples of H.pylori including20 clinical isolates and 1 reference strain NCTC 14126

but it was negative for other bacterialspecies

showing the N-PCR assay to be 100% specific.Tenfold serial dilution experiments re-vealed the detection of as little as 0.1 fg of H.pylori DNA by N-PCR.To evaluate the PCR as-say for clinical samples

gastric biopsies were tested with N-PCR

and the results were comparedwith those of culture

urease test and histologic examination（reference standard

RS）.In 30biopsy specimens

H.pylori DNA sequences were detected by PCR in all of 20（100%）positivetissue and none of the 10 negative tissues.PCR is a specific and sensitive method that can detectthe presence of H.pylori without the need for culture and would have significant importance di-agnostically and epidemiologically.

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据