Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction

杨立宏; 苏成芝

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Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction

Updated：2026-03-12

- Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction
- Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction
- 解放军医学杂志（英文版） 1992年第3期页码：222-226
- Affiliations：
  
  1. Department
  2. Department of Biochemistry
  3. Fourth Military Medical University
  4. ,Xi’an,710032
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1992
- Accepted：
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Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction[J]. 解放军医学杂志（英文版）, 1992,(3):222-226.

[1]杨立宏,苏成芝.Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction[J].Journal of Medical Colleges of PLA,1992(03):222-226.
Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction[J]. 解放军医学杂志（英文版）, 1992,(3):222-226. DOI：

[1]杨立宏,苏成芝.Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction[J].Journal of Medical Colleges of PLA,1992(03):222-226. DOI：

摘要

Abstract

<正> One oligonueleotide probe

HIV env 101-1

and two oligonucleotide primers

HIV env125-1 and HIV env 125-2

were designed with the aid of a computer and synthesized by a DNAsynthesizer.The env 125 peptide gene was amplified by polymerase chain reaetion（PCR）.Beingidentified by electrophoresis and Southern blotting

the PCR product was cloned into plasmidpUC 19.The recombinant pENV 125

identified with X-gal selection and restriction mapping

wassequenced.The results showed that the cloned env 125 peptide gene contained the inserted EcoRⅠ site and ATG at the 5’end

Hind Ⅲ site and TAG at the 3’end.The sequence and readingframe were proved to be correct.

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