Chemical and enzymatic synthesis and doning of human epidermal growth factor gene

赵忠良; 陈南春; 李飚; 陈苏民

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Chemical and enzymatic synthesis and doning of human epidermal growth factor gene

Updated：2026-03-12

- Chemical and enzymatic synthesis and doning of human epidermal growth factor gene
- Chemical and enzymatic synthesis and doning of human epidermal growth factor gene
- 解放军医学杂志（英文版） 1992年第2期页码：146-148
- Affiliations：
  
  1. Department of Biochemistry Fourth Military Medical University
  2. ,Xi’an,710032
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1992
- Accepted：
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Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J]. 解放军医学杂志（英文版）, 1992,(2):146-148.

[1]赵忠良,陈南春,李飚,陈苏民.Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J].Journal of Medical Colleges of PLA,1992(02):146-148+157.
Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J]. 解放军医学杂志（英文版）, 1992,(2):146-148. DOI：

[1]赵忠良,陈南春,李飚,陈苏民.Chemical and enzymatic synthesis and doning of human epidermal growth factor gene[J].Journal of Medical Colleges of PLA,1992(02):146-148+157. DOI：

摘要

Abstract

<正> The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites

andto elhninate the repeated and self-complementary sequences.So that multiple doning and gene ex-pression were available for the designed gene sequence.The whole gene was formed by annealing8 chemically synthesized oligodeoxynucleotide fragments

enzymatically filling up the 4 twenty-basespaces and ligating the nicks

and was inserted into EcoR I and HInc Ⅱ sites ofplasmid pUC12.The recombinant plasmid was transformed into E.coli JM103 and 200 whitedones were obtained by X-gal and ampicilin screening.The positive clones were isolated andcharacterized by restriction enzyme analysis and DNA sequencing.

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