Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period

李纪良; 李英杰; 巢穗; 欧阳明辉; 彭一兵

Your Location：

Home >

Browse articles >

Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period

Updated：2026-03-12

- Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period
- Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period
- 解放军医学杂志（英文版） 1992年第3期页码：246-250
- Affiliations：
  
  1. Department of Malaria Immunology First Military Medical University
  2. ,Guangzhou,510515
- Author bio：
- Funds：
  
  Partially supported by grants from the National Natural Science Foundation of China（No.3880715）;General Logistics Department of PLA;and the President Foundation of the First Military Medical University
- DOI：
  中图分类号：
- 纸质出版：1992
- Accepted：
Scan QR Code
Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period[J]. 解放军医学杂志（英文版）, 1992,(3):246-250.

[1]李纪良,李英杰,巢穗,欧阳明辉,彭一兵.Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period[J].Journal of Medical Colleges of PLA,1992(03):246-250.
Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period[J]. 解放军医学杂志（英文版）, 1992,(3):246-250. DOI：

[1]李纪良,李英杰,巢穗,欧阳明辉,彭一兵.Some biological characteristics of hybridomas and parental myelomas cultivated in serum-free medium for long-term period[J].Journal of Medical Colleges of PLA,1992(03):246-250. DOI：

摘要

Abstract

<正> It has been developed in this laboratory that a serum-free medium

designated asDMI

is adaptive for long-term culture

freezing and resurgence in liquid nitrogen

and cloningof hybridoma and parental myelomas as well as for cell fusions.In this report

it is describedthat the myeloma cells grown in DMI for more than 3 months maintained their biological charac-teristics such as induction of aseites and subcutaneous somatic tumor in BALB/c mice and toler-ance toward 8-azaguanine

ete..However

the ability of the tumor cells to attach to glass wallwas decreased.The selecting assay of these cells in HAT medium（medium containing hypoxan-thine

aminopterin and thymidine）showed that the death time in DMI was similar to that in theconventional 15％ newborn calf serum-supplemented medium（RPS15）.The hybridoma cellsadapted in DMI secrete monoclonal antibodies in quantities comparable to those produced inRPS15.

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据