Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus

戚中田; 潘卫; 杜平

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Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus

Updated：2026-03-12

- Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus
- Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus
- 解放军医学杂志（英文版） 1993年第2期页码：103-108
- Affiliations：
  
  1. Department of Microbiology Second Military Medical University
  2. ,Shanghai,200433
- Author bio：
- Funds：
- DOI：
  中图分类号：
- 纸质出版：1993
- Accepted：
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Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus[J]. 解放军医学杂志（英文版）, 1993,(2):103-108.

[1]戚中田,潘卫,杜平.Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus[J].Journal of Medical Colleges of PLA,1993(02):103-108.
Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus[J]. 解放军医学杂志（英文版）, 1993,(2):103-108. DOI：

[1]戚中田,潘卫,杜平.Cloning and sequencing of core gene cDNA of Chinese hepatitis C virus[J].Journal of Medical Colleges of PLA,1993(02):103-108. DOI：

摘要

Abstract

<正> A hepatitis C virus（HCV）cDNA fragment

534bp in length and designated asQ534

was obtained by PCR amplification with self-designed primers.Q534 was cloned in-to Hinc Ⅱ site of pUC18 and the recombinant plasmid pQ534 was then selected from thebacterial transformants.The sequence analysis indicated that Q534 was a cDNA fragmentof HCV core gene

and located in HCV genome from positions 320 to 853 incorrespondence with Chiron’s prototype sequence.The homologies between Q534 and theprototype at the levels of nucleotides and amino acids were 90.0% and 97.6%

respectively.The homologies of Q534 with Japanese HCV-J and HCV-BK strains were 96.6% and97.0% at the nucleotide level

and 98.2% and 98.8% at the amino acid level.In terms ofthe sequence

this Chinese HCV isolate should belong to HCV group Ⅱ.

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