Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining

卫立辛; 闫振林; 沈锋; 谢天培; 钱其军; 崔贞福; 郭亚军; 吴孟超

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Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining

Updated：2026-03-12

- Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining
- Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining
- 解放军医学杂志（英文版） 1998年第2期页码：140-142
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  中图分类号： R329.2
- 纸质出版：1998
- Accepted：
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Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining[J]. 解放军医学杂志（英文版）, 1998,(2):140-142.

[1]卫立辛,闫振林,沈锋,谢天培,钱其军,崔贞福,郭亚军,吴孟超.Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining[J].Journal of Medical Colleges of PLA,1998(02):140-142.
Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining[J]. 解放军医学杂志（英文版）, 1998,(2):140-142. DOI：

[1]卫立辛,闫振林,沈锋,谢天培,钱其军,崔贞福,郭亚军,吴孟超.Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining[J].Journal of Medical Colleges of PLA,1998(02):140-142. DOI：

摘要

Abstract

Telomerase is a ribonucleoprotein enzyme

which synthesizes telomeric repeats （TTAGGG） n.While germline cells and most malignant tumor cells express telomerase activity

normal somatic cells aregenerally deficient in telomerase activity. Our objective was to detect telomerase activity of human cells bysilver staining with telorneric repeat amplification protocol （TRAP） which is easy and quick. Comparing withradioisotopic TRAP

we examined the telomerase activity in telomerase-positive 293-cell and RNase-pretreated and heat-pretreated negative controls by silver staining TRAP. We detected telomerase activity in 2 strainsof human liver tumor cells （QGY7701 and SMMC7721 ）. The 293 cells （only 10 cells） and the 2 strains ofhuman liver tumor cells were all positive. while telomerase activity was not detected in the negative controls.These data suggest that non-radioisotopic silver staining TRAP is a specific

sensitive and fast assay fortelomerase activity. It was verified that the 2 strains of human liver tumor cells express telomerase activity.

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