Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration

刘厚奇; 喻德华; 汤瑞宝; 戚中田; 冯根生

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Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration

Updated：2026-03-12

- Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration
- Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration
- 解放军医学杂志（英文版） 2002年第4期页码：260-263
- Affiliations：
  
  1. Department of Histology & Embryology
  2. College of Basic Medical Sciences
  3. Second Military Medical University
  4. ,China
  5. China Lab of China-US Molecule Development
- Author bio：
- Funds：
- DOI：
  中图分类号： R321
- 纸质出版：2002
- Accepted：
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Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration[J]. 解放军医学杂志（英文版）, 2002,(4):260-263.

[1]刘厚奇,喻德华,汤瑞宝,戚中田,冯根生.Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration[J].Journal of Medical Colleges of PLA,2002(04):260-263.
Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration[J]. 解放军医学杂志（英文版）, 2002,(4):260-263. DOI：

[1]刘厚奇,喻德华,汤瑞宝,戚中田,冯根生.Involvement of Shp-2 phosphatase in IL-1α mediated mouse embryo fi- broblast MMP-1 secretion and migration[J].Journal of Medical Colleges of PLA,2002(04):260-263. DOI：

摘要

Abstract

<正> Objective: To investigate the relation between Shp-2 （a cytoplasmic tyrosine phosphotase） and matrix metalloproteinase-1 （MMP-1） in the migration of mouse embryo fibroblast cell. Methods: Shp-2 -/-embryo fibroblast was separated from Shp-2 knockout mouse on E10. 5

and Shp-2 cDNA was transfected into Shp-2 -/- cell. The cell migration was observed with the wound healing

the MMP-1 expression and secretion was analyzed by immunoblotting and immunoprecipitation. The activation of MMP-1 in the supernatant was detected with type I collagenase activity assay after the stimulation of IL-1α compared with E10. 5 mouse embryo fibroblast cell（Shp-2 +/+）. Results: There was obvious migration in Shp-2 +/+ cells and Shp-2 -/-R cells

but not in Shp-2 -/- cells. The shape of Shp-2 -/- cell was epithelial-like. The expression and secretion was increased in Shp-2 +/+ cells and Shp-2 -/-R cells

and it had not changed in Shp-2 -/- cell. The activation of MMP-1 was lower in Shp-2 -/- cells compared to the other cells. C

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