Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction

胡子有; 马文丽; 宋艳斌; 张宝; 吴清华; 郭秋野; 彭翼飞; 郑文岭

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Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction

Updated：2026-03-12

- Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction
- Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction
- 解放军医学杂志（英文版） 2002年第4期页码：276-280
- Affiliations：
  
  1. Institute of Molecular Biology
  2. First Military Medical University
  3. ,Guangzhou,510010
  4. Institute of Molecular Oncology
  5. Liuhuaqiao Hospital
- Author bio：
- Funds：
- DOI：
  中图分类号： R346
- 纸质出版：2002
- Accepted：
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Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction[J]. 解放军医学杂志（英文版）, 2002,(4):276-280.

[1]胡子有,马文丽,宋艳斌,张宝,吴清华,郭秋野,彭翼飞,郑文岭.Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction[J].Journal of Medical Colleges of PLA,2002(04):276-280.
Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction[J]. 解放军医学杂志（英文版）, 2002,(4):276-280. DOI：

[1]胡子有,马文丽,宋艳斌,张宝,吴清华,郭秋野,彭翼飞,郑文岭.Cloning of polyadenylated mRNA fragments of Escherichia coli with restriction display-polymerase chain reaction[J].Journal of Medical Colleges of PLA,2002(04):276-280. DOI：

摘要

Abstract

<正> Objective: To investigate the polyadenylation of mRNA in E. coli. Methods: The mRNA of E. coli was enriched from the total RNA with oligo（dT）-cellulose

prior to reverse transcription using oligo（dT）18 as the primer. Double-stranded cDNA was subsequently synthesized

which was subjected to digestion with Sau3A. I to produce multiple gene fragments for ligation with the adapters. PCR was carried out in 10 groups according to 10 different pairs of the selective primers

and the PCR products were then cloned into T-vectors. Results: More than 100 gene fragments had been cloned

30 of which were sequenced. Conclusion: Polyadenylation of E. coli mRNA may not be a biochemical curiosity but a general attribute of bacterial mRNA.

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