Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca<sup>2+</sup>

周舟; 王小华; Igisu Hideki; 林远; 楼淑芬; Matsuoka Masato; 程天民; 余争平

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Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺

Updated：2026-03-12

- Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺
- Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺
- 解放军医学杂志（英文版） 2002年第3期页码：181-187
- Affiliations：
  
  1. Institute of Combined Injury
  2. Third Military Medical University
  3. ,Japan
  4. Department of Environmental Toxicology
  5. University of Occupational and Environmental Health
  6. Kitakyushu 5,807-8555
- Author bio：
- Funds：
  
  in part by Natural Sciences Foundation of China （No. 39870239）;by the Sasagawa Fellowship,Japan.
- DOI：
  中图分类号： R363
- 纸质出版：2002
- Accepted：
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Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺[J]. 解放军医学杂志（英文版）, 2002,(3):181-187.

[1]周舟,王小华,Igisu Hideki,林远,楼淑芬,Matsuoka Masato,程天民,余争平.Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca~(2+)[J].Journal of Medical Colleges of PLA,2002(03):181-187.
Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺[J]. 解放军医学杂志（英文版）, 2002,(3):181-187. DOI：

[1]周舟,王小华,Igisu Hideki,林远,楼淑芬,Matsuoka Masato,程天民,余争平.Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca~(2+)[J].Journal of Medical Colleges of PLA,2002(03):181-187. DOI：

摘要

Abstract

<正> Objective: To explore the effects of γ-irradiation on mitogen-activated protein kinases （MAPKs） and role of intracellular calcium in this event in intestinal epithelial cell line 6 （IEC-6 cells）. Methods: After cultured rat IIEC-6 cells with or without the pretreatment of intracellular Ca2+ chelator were exposed to Y-ir-radiation of 6 Gy

the total and phosphorylated MAPKs in the cells were determined with Western blotting and apoptosis was examined with flow cytometry. Activities of Extracellular signal-regulated protein kinase （ERK） and p38 MAPK were determined by using immuoprecipitation followed by Western blotting. Results: In response to γ-irradiation

phosphorylation of ERK was not significantly observed

while the levels of phosphorylated c-Jun NH2-terminal kinase （JNK） and p38 MAPK were increased in 30 min and reached the peak 2 h after exposure to 6 Gy γ-irradiation

though the cell viability was significantly lowered 12 h. On the other hand

no obvious changes were seen in the total protei

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Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca2+

Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca2+

DOI：

摘要

Abstract

关键词

Keywords

references

Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺

Differential activation of mitogen-activated protein kinases by γ-irradi-ation in IEC-6 cells: Role of intracellular Ca²⁺