Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells

阚文宏; 闫文生; 姜勇; 王静珍; 秦清和; 赵克森

Your Location：

Home >

Browse articles >

Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells

Updated：2026-03-12

- Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells
- Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells
- 解放军医学杂志（英文版） 2002年第4期页码：235-241
- Affiliations：
  
  1. Department of Pathophysiology
  2. First Military Medical University
  3. ,China
- Author bio：
- Funds：
  
  State Key Development Program of Basic Research （No. G2000057004）;Key Project of National Natural Science Foundation （No 39830400）;National Natural Science Foundation of China （No. 30070735）;Key Project of Science and Technology of Guangdong Provin
- DOI：
  中图分类号： R363
- 纸质出版：2002
- Accepted：
Scan QR Code
Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells[J]. 解放军医学杂志（英文版）, 2002,(4):235-241.

[1]阚文宏,闫文生,姜勇,王静珍,秦清和,赵克森.Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells[J].Journal of Medical Colleges of PLA,2002(04):235-241.
Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells[J]. 解放军医学杂志（英文版）, 2002,(4):235-241. DOI：

[1]阚文宏,闫文生,姜勇,王静珍,秦清和,赵克森.Role of p38 MAPK in lipopolysaccharide-induced iNOS expression by endothelial cells[J].Journal of Medical Colleges of PLA,2002(04):235-241. DOI：

摘要

Abstract

<正> Objective: To examine the role of p38 mitogen-activated protein kinase （MAPK） in NO production and iNOS expression in human endothelial cells stimulated by lipopolysaccharide （LPS）. Methods: The NO level in the supernatant of the cell culture media was measured with Griess method

expressions of iNOS protein and mRNA in vitro cultured endothelial cell line ECV304 were detected with immunofluorescence analysis and reverse transcriptase-PCR respectively. Immunokinase assay was employed to measure p38 MAPK activity. Results: Compared with the basal level of iNOS expression and NO production

the NO level and the expressions of iNOS mRNA and protein in the cells were increased after LPS stimulation. p38 MAPK activity in ECV304 cells exhibited a marked increase at 15 min after LPS stimulation

lasting for about 45 min before gradually decline. The iNOS protein and mRNA expressions induced by LPS stimulation was significantly inhibited by SB203580 [4-（4-fluorophenyl）-2-（4- methylsulfinylphenyl）-5-（4-pyridyl）

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据