Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism

施理; 孙勇; 张亚力; 张振书; 周殿元

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Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism

Updated：2026-03-12

- Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism
- Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism
- 解放军医学杂志（英文版） 2002年第1期页码：14-16
- Affiliations：
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China （No. 39570334）
- DOI：
  中图分类号： R446.5
- 纸质出版：2002
- Accepted：
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Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism[J]. 解放军医学杂志（英文版）, 2002,(1):14-16.

[1]施理,,,,,,,,,,孙勇,,,,,,,,,,张亚力,,,,,,,,,,张振书,,,,,,,,,,周殿元.Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism[J].Journal of Medical Colleges of PLA,2002(01):14-16+33.
Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism[J]. 解放军医学杂志（英文版）, 2002,(1):14-16. DOI：

[1]施理,,,,,,,,,,孙勇,,,,,,,,,,张亚力,,,,,,,,,,张振书,,,,,,,,,,周殿元.Differentiation of Helicobacter pylori isolates by polymerase chain reaction-restriction fragment length polymorphism[J].Journal of Medical Colleges of PLA,2002(01):14-16+33. DOI：

摘要

Abstract

<正>Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori （H. pylori）. Methods: Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4 types according to their PCR-RFLP results of urease gene and urease activity. Type Ⅰ

possessing strong urease activity （0. 11） and presented 1 fragment of 1. 7 kb by PCR-RFLP

had close relations with gastric ulcer; type Ⅱ

with the weakest urease activity （0. 07） and 2 fragments （1. 3 and 0. 4 kb respectively）

was associated with duodenal bulb ulcer; type Ⅲ

with the strongest urease activity （0. 12） and 2 fragments （0. 4 and 0. 17 kb） with or without 1 fragment （0. 23 or 0. 37 kb）

was responsible for gastritis; type Ⅳ

with weak urease activity （0. 09） and 2 fragments

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