Analysis of gene expression profile of aspermia using cDNA microarray

杨波; 高晓康; 王禾; 刘贺亮; 陈宝琦; 秦荣良; 康福霞; 邵国兴; 邵晨

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Analysis of gene expression profile of aspermia using cDNA microarray

Updated：2026-03-12

- Analysis of gene expression profile of aspermia using cDNA microarray
- Analysis of gene expression profile of aspermia using cDNA microarray
- 解放军医学杂志（英文版） 2003年第4期页码：237-241
- Affiliations：
  
  1. Department of Urology
  2. Xijing Hospital
  3. Fourth Military Medical University
  4. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R346
- 纸质出版：2003
- Accepted：
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Analysis of gene expression profile of aspermia using cDNA microarray[J]. 解放军医学杂志（英文版）, 2003,(4):237-241.

[1]杨波,高晓康,王禾,刘贺亮,陈宝琦,秦荣良,康福霞,邵国兴,邵晨.Analysis of gene expression profile of aspermia using cDNA microarray[J].Journal of Medical Colleges of PLA,2003(04):237-241.
Analysis of gene expression profile of aspermia using cDNA microarray[J]. 解放军医学杂志（英文版）, 2003,(4):237-241. DOI：

[1]杨波,高晓康,王禾,刘贺亮,陈宝琦,秦荣良,康福霞,邵国兴,邵晨.Analysis of gene expression profile of aspermia using cDNA microarray[J].Journal of Medical Colleges of PLA,2003(04):237-241. DOI：

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Abstract

<正> Objective: To identify the differential gene expression profiles between the normal and aspermia human testes utilizing cDNA microarray. Methods: cDNA probes were prepared by labeling mRNA of aspermia testes tissues with Cy5-dUTP and mRNA of normal testes tissues with Cy3-dUTP respectively through reverse transcription. The mixed cDNA probes were then hybridized with 4096 cDNA arrays （4096 unique human cDNA sequences）

and the fluorescent signals were scanned by ScanArray 3000 scanner （General Scanning

Inc. ）. The values of Cy5-dUTP and Cy3-dUTP on each spot were analyzed and calculated by ImaGene 3. 0 software （ BioDiscovery

Inc. ）. Differentially expressed genes were screened according to the criterion that the absolute value of natural logarithm of the ratio of Cy5-dUTP to Cy3-dUTP was greater-than 2. 0 or less-than 0. 5. A randomly chosen gene RAP1A was studied by in situ hybridization to evaluate the accuracy of the results. Results: 623 differential expressed genes related to aspermia were foun

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