Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity

全松; 周海宽; 山野修司; 中坂尚代; 青野敏博

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Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity

Updated：2026-03-12

- Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity
- Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity
- 解放军医学杂志（英文版） 2003年第1期页码：51-55
- Affiliations：
  
  1. Department of Obstetrics & Gynecology
  2. Nanfang Hospital
  3. First Military Medical University
  4. Department of Urological Surgery
  5. School of Medicine
  6. the University of Tokushima
  7. the University of Tokushima Guangzhou
  8. ,Japan
- Author bio：
- Funds：
- DOI：
  中图分类号： R321
- 纸质出版：2003
- Accepted：
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Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity[J]. 解放军医学杂志（英文版）, 2003,(1):51-55.

[1]全松,周海宽,山野修司,中坂尚代,青野敏博.Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity[J].Journal of Medical Colleges of PLA,2003(01):51-55.
Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity[J]. 解放军医学杂志（英文版）, 2003,(1):51-55. DOI：

[1]全松,周海宽,山野修司,中坂尚代,青野敏博.Cold-preserved human spermatozoa in electrolyte-free solution retain their pene-tration capacity[J].Journal of Medical Colleges of PLA,2003(01):51-55. DOI：

摘要

Abstract

<正> Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free （EF） solution at 4 ℃. Methods: The motility

acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution

respectively. Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid （ mHTF）（43. 4% ± 7. 9% vs 9. 5% ±2. 5%

P <0. 01 ）. Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm （ capacitated sperm; 7. 6% ± 1. 8% vs6.4±1.8%; acrosome-reacted sperm; 3.0% ±1.7% vs2.4±1.1%

P>0.05）

the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation （capacitated sperm; 16. 0% ± 2.3%vs7.6±1.8%

acrosome-reacted sperm: 9. 4% ±2.1% vs 3. 0% ±1.7%

P<0.01）. The penetration rate and fe

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