<正> Objective: To establish a novel approach for quick and highly efficient verification of human gene imprinting. Methods: A pair of dye-labelled probes

5’ nuclease assay was combined with RT-PCR to determine the genotype of a transcribed single nucleotide polymorphism （SNP） rs705（C>T） of a known imprinted gene

small nuclear ribonucleotide protein N （SNRPN）

on both genomic DNA and cDNA of human lym-phoblast cell lines. Results: Allele discrimination showed a clear monoallelic expression pattern of SNRPN

which was confirmed by RT-PCR based restriction fragment length polymorphism （RFLPs）. Pedigree analysis verified the paternal origin of expressed allele

which was in consistency with previous report. Conclusion: Transcribed SNP is an ideal marker for detecting gene imprinting by 5’ nuclease assay. This approach also may be used to discover differential allele expression of non-imprinted genes

finding out gene cis-acting functional polymorphism.