Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721

牟娇; 李晓冬; 杨庆; 贾凤岐; 卫立辛; 郭亚军; 吴孟超

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Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721

Updated：2026-03-12

- Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721
- Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721
- 解放军医学杂志（英文版） 2003年第3期页码：170-174
- Affiliations：
  
  1. Cancer Institute
  2. Second Military Medical University
  3. ,China
  4. Tumor Immunology and Gene Therapy Center
  5. Eastern Hopatobiliary Surgery Hospital
  6. Eastern Hepatobiliary Surgery Hospital
- Author bio：
- Funds：
  
  Supported by National 973 Project of China（No.G2000057001）
- DOI：
  中图分类号： R735.7
- 纸质出版：2003
- Accepted：
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Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721[J]. 解放军医学杂志（英文版）, 2003,(3):170-174.

[1]牟娇,李晓冬,杨庆,贾凤岐,卫立辛,郭亚军,吴孟超.Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721[J].Journal of Medical Colleges of PLA,2003(03):170-174.
Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721[J]. 解放军医学杂志（英文版）, 2003,(3):170-174. DOI：

[1]牟娇,李晓冬,杨庆,贾凤岐,卫立辛,郭亚军,吴孟超.Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721[J].Journal of Medical Colleges of PLA,2003(03):170-174. DOI：

摘要

Abstract

<正> Objective: To induce changes in biological character of human liver cancer cell line SMMC-7721 by bloc-king the expression of telomerase genes hTRT and to explore its value in cancer gene therapy. Methods: The vehiclefor eukaryotic expression of antisense hTRT was constructed and then transfected into SMMC-7721 cells. The effects ofantisense hTRT gene on telomerase activity

cancer cell growth and malignant phenotypes were analyzed. Results: Theobtained transfectants that could express antisense hTRT gene stably showed marked decrease in telomerase activity;the shortening of telomere was obvious; cells presented contact growth inhibition; in nude mice transplantation

the rateof tumor induction dramatically decreased. Conclusion: Antisense hTRT gene expression can significantly inhibit te-lomerase activity of cancer cells and decrease malignant phenotypes in vitro and in vivo. Therefore

as a telomerase in-hibitor

antisense hTRT gene may be a new pathway for cancer therapy.

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