Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit

陆翠华; 陈岳祥; 张忠兵; 谢渭芬; 张兴荣; 卫立辛; 郭亚军; 金由辛

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Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit

Updated：2026-03-12

- Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit
- Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit
- 解放军医学杂志（英文版） 2002年第1期页码：21-23
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China （No. 39870303）
- DOI：
  中图分类号： R346
- 纸质出版：2002
- Accepted：
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Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit[J]. 解放军医学杂志（英文版）, 2002,(1):21-23.

[1]陆翠华,,,,,,,,,,陈岳祥,,,,,,,,,,张忠兵,,,,,,,,,,谢渭芬,,,,,,,,,,张兴荣,,,,,,,,,,卫立辛,,,,,,,,,,郭亚军,,,,,,,,,,金由辛.Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit[J].Journal of Medical Colleges of PLA,2002(01):21-23+59.
Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit[J]. 解放军医学杂志（英文版）, 2002,(1):21-23. DOI：

[1]陆翠华,,,,,,,,,,陈岳祥,,,,,,,,,,张忠兵,,,,,,,,,,谢渭芬,,,,,,,,,,张兴荣,,,,,,,,,,卫立辛,,,,,,,,,,郭亚军,,,,,,,,,,金由辛.Comparison of cleavage activity in vitro between two ribozymes targeted against different sites of PDGF receptor β subunit[J].Journal of Medical Colleges of PLA,2002(01):21-23+59. DOI：

摘要

Abstract

<正>Objective: To compare the cleavage activity of ribozymes directed against 2 sites of PDGF receptorP subunit cDNA gene in vitro. Methods: The 608 bp fragment of PDGF receptor β subunit cDNA was clonedinto T-vector under the control of T7 promoter

named pPDGFR-β. Two ribozymes were designed to cleavethe CUU sequence at codon 45 and codon 252 of PDGF receptor β subunit mRNA respectively. These 2 ham-merhead ribozyme genes were cloned into vector PI. 5 between 5’ -cis ribozyme and 3’ -cis ribozyme to gener-ate the plasmids of pRZ1 and pRZ2. The pPDGFR-β

pRZl and pRZ2 were linearized and then transcribedwith T7 promoter in vitro. Results: The RZ1 showed high cleavage activity in vitro

but the RZ2 showed nocleavage activity under the same condition. Conclusion: The cleavage site selection is an important factor in-fluencing the cleavage activities of ribozymes.

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