Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene

何平; 孙刚

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Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene

Updated：2026-03-12

- Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene
- Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene
- 解放军医学杂志（英文版） 2003年第3期页码：150-153
- Affiliations：
  
  1. Department of Physiology
  2. College of Basic Medical Sciences
  3. Second Military Medical University
  4. ,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China（No.39970285）,Shanghai Science and Technology Development Project（No.99JC14036）
- DOI：
  中图分类号： R335
- 纸质出版：2003
- Accepted：
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Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene[J]. 解放军医学杂志（英文版）, 2003,(3):150-153.

[1]何平,孙刚.Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene[J].Journal of Medical Colleges of PLA,2003(03):150-153.
Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene[J]. 解放军医学杂志（英文版）, 2003,(3):150-153. DOI：

[1]何平,孙刚.Effect of glucocorticoid on promoter of 11β-hydroxysteroid dehydrogenase Ⅱ gene[J].Journal of Medical Colleges of PLA,2003(03):150-153. DOI：

摘要

Abstract

<正> Objective: To study the effect of glucocorticoid on the promoter of the pre-receptor glucocorticoid metabo-lizing enzyme 11β-hydroxysteroid dehydrogenase type 1（11β-HSD1）gene. Methods: The 1.2 kb length sequenceupstream to the transcription start site of the 11β-HSD1 gene was amplified with polymerase chain reaction （PCR） andthen was cloned into pBLCAT6 plasmid carrying chloramphenicol acetyltransferase （CAT） reporter gene. The plasmidpBLCAT6 carrying the promoter and reporter gene was used to transfect HeLa cells to study the regulation of 11β-HSD1gene expression by glucocorticoids in terms of reporter gene expression. Results: PCR showed that there was a com-plete alignment of the amplified sequence with the sequence 1.2 kb upstream to the transcription start site of 11β-HSD1gene. When cloned into pBLCAT6 plasmid carrying the reporter gene

this part of the promoter is functional in terms ofregulation of reporter gene expression upon transfection into HeLa cells. The synthetic glucocorticoid-dexa

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