Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats

湛冯岚; 袁伟杰; 梅小斌; 吴灏; 许静; 刘宇建; 卢建

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Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats

Updated：2026-03-12

- Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats
- Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats
- 解放军医学杂志（英文版） 2002年第3期页码：171-173
- Affiliations：
  
  1. Department of Nephrology
  2. Changhai Hospital
  3. Second Military Medical University
  4. ,China
  5. Department of Pathophysiology
- Author bio：
- Funds：
- DOI：
  中图分类号： R692
- 纸质出版：2002
- Accepted：
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Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats[J]. 解放军医学杂志（英文版）, 2002,(3):171-173.

[1]湛冯岚,袁伟杰,梅小斌,吴灏,许静,刘宇建,卢建.Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats[J].Journal of Medical Colleges of PLA,2002(03):171-173+217.
Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats[J]. 解放军医学杂志（英文版）, 2002,(3):171-173. DOI：

[1]湛冯岚,袁伟杰,梅小斌,吴灏,许静,刘宇建,卢建.Parathyroid hormone stimulating synthesis of fibronectin by mesangial cells via TGF-β in rats[J].Journal of Medical Colleges of PLA,2002(03):171-173+217. DOI：

摘要

Abstract

<正> Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin （FN） from cultured rat mesangial cells and its possible mechanism. Methods: （1） MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34（10-12 mol/l -10-8 mol/l） for 6 h

12 h

24 h and 48 h

control cells were treated with vehicle only. The FN levels （in the supernatant） were measured by ELISA assay. （2） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34（10-12 mol/l - 10-8 mol/l ）. Forty-eight hours later

FN were tested by ELISA. （4） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h

12 h

24 h and 48 h and then FN were tested. Results: （1） hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l （P<0. 01）. （2） Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34

on synthesis of FN in cultured rat mesangial cells

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