Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro

陆俊羽; 姚伟; 钱桂生; 吴国明

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Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro

Updated：2026-03-12

- Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro
- Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro
- 解放军医学杂志（英文版） 2002年第4期页码：264-269
- Affiliations：
  
  1. Institute of Respiratory Diseases
  2. Xinqiao Hospital
  3. Third Military Medical University
  4. ,China
- Author bio：
- Funds：
  
  National Natural Science Foundation of China （No. 39870352）
- DOI：
  中图分类号： R543.2
- 纸质出版：2002
- Accepted：
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Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro[J]. 解放军医学杂志（英文版）, 2002,(4):264-269.

[1]陆俊羽,姚伟,钱桂生,吴国明.Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro[J].Journal of Medical Colleges of PLA,2002(04):264-269+275.
Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro[J]. 解放军医学杂志（英文版）, 2002,(4):264-269. DOI：

[1]陆俊羽,姚伟,钱桂生,吴国明.Effects of NHE-1 ribozyme gene transfection on apoptosis of rat pulmonary artery smooth muscle cells in vitro[J].Journal of Medical Colleges of PLA,2002(04):264-269+275. DOI：

摘要

Abstract

<正> Objective : To investigate the effects of the transfection of NHE-1 ribozyme gene on the apoptosis of pulmonary artery smooth muscle cells （PASMC） in vitro. Methods: After NHE-1 ribozyme gene was designed

synthesized and then cloned into plasmid pLXSN

the recombined plasmid was tansfected into cultured rat PASMC. Expression of NHE-1 mRNA was detected with semi-quantitative RT-PCR. Intracellular pH （pHi） was measured by using fluorescence dye BCECF-AM. Cell cycle was measured with aid of flow cy-tometric DNA analysis. Cell apoptosis was observed with electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNED respectively. Results: The NHE-1 mRNA expression level and pHi value were significantly lower in PASMCs transfected with NHE-1 ribozyme gene than those transfected with pLXSN or without transfection. Meanwhile

the apoptosis rate of cells transfected with NHE-1 ribozyme gene was increased significantly. Morphology of cell apoptosis was observed in the cell

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