Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction

刘胜学; 曹佳; 安辉

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Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction

Updated：2026-03-12

- Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction
- Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction
- 解放军医学杂志（英文版） 2002年第2期页码：105-111
- Affiliations：
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation （No. 39970650） and Chongqing Public Health Bureau Foundation （2000）.
- DOI：
  中图分类号： R733.7
- 纸质出版：2002
- Accepted：
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Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction[J]. 解放军医学杂志（英文版）, 2002,(2):105-111.

[1]刘胜学,,,,,,,,,,曹佳,,,,,,,,,,安辉.Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction[J].Journal of Medical Colleges of PLA,2002(02):105-111.
Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction[J]. 解放军医学杂志（英文版）, 2002,(2):105-111. DOI：

[1]刘胜学,,,,,,,,,,曹佳,,,,,,,,,,安辉.Molecular analysis of THH-induced mutations at HPRT locus in human promyelocytic leukemia cells with multiplex polymerase chain reaction[J].Journal of Medical Colleges of PLA,2002(02):105-111. DOI：

摘要

Abstract

<正>Objective: To study the genotoxicity and antitumor activity of a Chinese medicinal herb

Triptery-gium Hypoglaucum （Level） Hutch （THH）. Methods: The genotoxicity and antitumor activity of THH were investigated in human promyelocytic leukemia cells on the mutation of hypoxanthine-guanine phosphoribosyl transferase （HPRT） gene by using single cell clone culture

two-way screening counting

multiplex PCR amplification and gel electrophoresis. Results: The results showed that different mutant spectra existed between the spontaneous mutation and induced mutation by THH. Only 7. 7% （1/13） of spontaneous mutants showed deletion mutations

whereas the induced mutants included 46. 6% （27/58） deletions. Mapping of all intra-genic deletion breakpoints showed a random distribution in all 9 exons

but toward the 3’-end of the HPRT gene. Deletion of exon 1 only appeared when whole gene was deleted. Deletions of exon 7/8 and 9 often showed linkage deletions （71. 4%）. Conclusion: THH can induce the mutation

mainly dele

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