Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant
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Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant
Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant
解放军医学杂志(英文版)2002年第2期 页码:85-87
Affiliations:
Author bio:
Funds:
Supported by the National Natural Science Foundation of China (No. 39870012)
DOI:
中图分类号:Q935
纸质出版:2002
Accepted:
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Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant[J]. 解放军医学杂志(英文版), 2002,(2):85-87.
[1]陈孙孝,,,,,,,,,,姚志荣,,,,,,,,,,温海,,,,,,,,,,邓安梅,,,,,,,,,,仲人前,,,,,,,,,,廖万清.Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant[J].Journal of Medical Colleges of PLA,2002(02):85-87+111.
Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant[J]. 解放军医学杂志(英文版), 2002,(2):85-87.DOI:
[1]陈孙孝,,,,,,,,,,姚志荣,,,,,,,,,,温海,,,,,,,,,,邓安梅,,,,,,,,,,仲人前,,,,,,,,,,廖万清.Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant[J].Journal of Medical Colleges of PLA,2002(02):85-87+111.DOI:
Study on growth cycle and ultrastructure of calmodulin fixed-point yeast mutant
摘要
Abstract
<正>Objective: To study the effects of the fixed-point mutant on growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast. Methods: Growth cycle and ultrastructure of the Calmodulin fixed-point mutant yeast were studied by using cytometry and scan electron microscope. Results: After culture at 30’C for 36 h
the content of aneuploidy of the temperature-sensitive recombinant yeast monoploid B(F92+) and E(F89+FH1 + ) strains were 63. 87% and 63. 55%
respectively. These were significantly different from control OP<0. 01) ; The content of the non-temperature-sensitive recombinant yeast monoploid A(F89+) and C(F141+) strains were 100. 00% and 99. 97%
respectively
which were not significantly different from control value (P>0. 05). Observed by scan electron microscope
the control group
A and C strain’s buds were spherical or oval and those bud-necks were fairly shorter; the B and E strain’s buds were slender
some cell were abnormal and some buds might show spherical or oval. Conclusion: DN
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