Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro

张勇; 邹仲敏; 郭朝华; 周进明; 王劲; 范文辉; 罗成基; 程天民

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Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro

Updated：2026-03-12

- Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro
- Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro
- 解放军医学杂志（英文版） 2003年第1期页码：27-31
- Affiliations：
  
  1. Department of Hematology
  2. Southwest Hospital
  3. Third Military Medical University
  4. Institute of Combined Injury of PLA
  5. Third Military Medical University Chongqing,400038
  6. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R329
- 纸质出版：2003
- Accepted：
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Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro[J]. 解放军医学杂志（英文版）, 2003,(1):27-31.

[1]张勇,邹仲敏,郭朝华,周进明,王劲,范文辉,罗成基,程天民.Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro[J].Journal of Medical Colleges of PLA,2003(01):27-31.
Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro[J]. 解放军医学杂志（英文版）, 2003,(1):27-31. DOI：

[1]张勇,邹仲敏,郭朝华,周进明,王劲,范文辉,罗成基,程天民.Experimental study on MyoD gene induced differentiation of bone marrow mesen-chymal stem cells into myoblasts in vitro[J].Journal of Medical Colleges of PLA,2003(01):27-31. DOI：

摘要

Abstract

<正> Objective: To explore the possibility of the transfection of MyoD gene induced bone marrow mesenchymal stem cells （ MSCs） to differentiate into myoblasts in vitro. Methods: The eukaryotic expression plasmid vector pIRES2-EGFP-MyoD was transfected into MSCs with lipotransfection method

and the positive cells were selected by G418; The expression of MyoD was detected in the transfected MSCs with RT-PCR and the amplified

purified product was identified by sequencing; The reporter gene enhanced green fluorescence protein （ EFGP） was observed in the transfected cells under a fluorescent and a laser confocal microscopes; Immunohistochemical methods was used to examine the expressions of MyoD

myogenin

myosin

myoglobin and desmin in the differentiated cells. The ultrastructure changes of the cells before and after transfection were observed with electron microscopy. Results: The expression of MyoD was detected in the transfected MSCs with RT-PCR and the amplified

purified product was as same in sequence a

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