Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells

李静; 刘陕西; 张梅

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Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells

Updated：2026-03-12

- Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells
- Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells
- 解放军医学杂志（英文版） 2003年第3期页码：165-169
- Affiliations：
  
  1. Department of Hematology
  2. First Hospital
  3. Xi’an Jiaotong University
  4. ,China
- Author bio：
- Funds：
  
  Supported by Xi’an Foundation of Science and Technology Program（200016）
- DOI：
  中图分类号： R73-3
- 纸质出版：2003
- Accepted：
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Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells[J]. 解放军医学杂志（英文版）, 2003,(3):165-169.

[1]李静,刘陕西,张梅.Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells[J].Journal of Medical Colleges of PLA,2003(03):165-169.
Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells[J]. 解放军医学杂志（英文版）, 2003,(3):165-169. DOI：

[1]李静,刘陕西,张梅.Effect of realgar on telomerase activity and hTERT-mRNA expression in NB4 cells[J].Journal of Medical Colleges of PLA,2003(03):165-169. DOI：

摘要

Abstract

正

Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase（hTERT） gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: Theexpression of hTERT-mRNA was analyzed by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）. Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay （PCR-ELISA）.Flow cytometry using PI staining was applied to analyze the cell cycle and apoptosis. Results: Treatment of NB4 cellswith 155

300

600 μg/L realgar reduced telomerase activity significantly accompanying with decrease of hTERT-mR-NA and increasing cell apoptosis. G2/M phase arrest appeared when treated with realgar in 300

600 μg/L. Condu-sion: It is suggested that telomerase activity of NB4 cells can be specifically inhibited by realgar through the down-regu-lation of hTERT gene expression. G

/M phase arrest and apoptosis by realgar in NB4 cells might be related to the re-ductio

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