TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine

杨佳荟; 沈茜

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TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine

Updated：2026-03-12

- TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine
- TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine
- 解放军医学杂志（英文版） 2003年第5期页码：301-304
- Affiliations：
  
  1. Department of Clinical Diagnosis
  2. Changhai Hospital
  3. Second Military Medical University
  4. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R542.2
- 纸质出版：2003
- Accepted：
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TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine[J]. 解放军医学杂志（英文版）, 2003,(5):301-304.

[1]杨佳荟,沈茜.TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine[J].Journal of Medical Colleges of PLA,2003(05):301-304.
TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine[J]. 解放军医学杂志（英文版）, 2003,(5):301-304. DOI：

[1]杨佳荟,沈茜.TaqMan real-time fluorescent quantitative RT-PCR in detection of macrophage inflammatory protein-2γ mRNA in myocarditis murine[J].Journal of Medical Colleges of PLA,2003(05):301-304. DOI：

摘要

Abstract

<正> Objective: To study the role of macrophage inflammatory protein （ MIP） -2γ in myocarditis pathogenesis in BALB/c mice. Methods: The relationship between the progression of Coxsarckie virus B3 （ CVB3 ） viral myocarditis and experimental autoimmune myocarditis and MIP-2γ mRNA expression in mouse was studied by TaqMan real-time fluorescent quantitative RT-PCR. Results: MIP-2γ mRNA expression rose on 3 to 5 d after CVB3 infection

reached peak on 7 d

and returned to normal level until 14 d

which corresponded well with the disease course. The MIP-2γ mRNA expression level rose significantly on the day 18 d after immunization with porcine cardiac myosin

which was consistent with pathological examination. Conclusion: MIP-2γ may be involved in the pathogenesis of myocarditis.

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