Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice
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Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice
Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice
解放军医学杂志(英文版)2003年第3期 页码:140-142
Affiliations:
1. Department of Histology & Embryology
2. School of Basic Medicine
3. Fourth Military Medical University
4. ,Beijing,100853
5. Department of Urinology
6. Xijing Hospital
7. Department of Neuron
8. Information Centre
9. No. General Hospital of Chinese PLA,301
Author bio:
Funds:
Supported by National Natural Science Foundation of China(No.39870109)
DOI:
中图分类号:R33
纸质出版:2003
Accepted:
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Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice[J]. 解放军医学杂志(英文版), 2003,(3):140-142.
[1]王红,张远强,孙岚,王春杨,尹岭.Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice[J].Journal of Medical Colleges of PLA,2003(03):140-142.
Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice[J]. 解放军医学杂志(英文版), 2003,(3):140-142.DOI:
[1]王红,张远强,孙岚,王春杨,尹岭.Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice[J].Journal of Medical Colleges of PLA,2003(03):140-142.DOI:
Improvement of mount preparations in showing myenteric nerve plexus from intestines of mice
摘要
Abstract
<正> Objective: The whole mount preparations of digestive tract is an effective experimental way to study the ap-pearance and distribution of nerve plexus in digestive tract. Although myentric nerve plexus preparations technique wasreported very early. But we have done experiment over and over during our research work in order to improve this tradi-tional method and to meet the needs of our research work
we made some progresses in regular mount preparations aftermany experiments
which helped offer better situation in observing myentric nerve plexus. Methods: Five healthy maleadult Kunming mice (20-30g in weight) were used in this study. After intraperitoneal injection of muscle relaxant
with dislocation of cervical vertebra method
the abdominal cavity was exposed through abdominal median incision. Af-ter several steps of mount preparations the mucous layer and longitudinal muscle layer mount preparations wih myentricnerve plexus were stripped under anatomical microscope. Immunohistochemical staining was a
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