Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells

郑燕芳; 张积仁

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Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells

Updated：2026-03-12

- Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells
- Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells
- 解放军医学杂志（英文版） 2003年第4期页码：262-266
- Affiliations：
  
  1. Department of Oncology
  2. Zhujiang Hospital
  3. First Military Medical University
  4. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R737.33
- 纸质出版：2003
- Accepted：
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Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells[J]. 解放军医学杂志（英文版）, 2003,(4):262-266.

[1]郑燕芳,张积仁.Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells[J].Journal of Medical Colleges of PLA,2003(04):262-266.
Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells[J]. 解放军医学杂志（英文版）, 2003,(4):262-266. DOI：

[1]郑燕芳,张积仁.Transfection with the ribozyme targeting HPVE6 mRNA results in growth inhibition of E6-expressing cervical carcinoma cells[J].Journal of Medical Colleges of PLA,2003(04):262-266. DOI：

摘要

Abstract

<正> Objective: To acquire a ribozyme against the E6 gene of human papillomaviruses type 16 （ HPV16E6） and investigate its effects on the phenotypes and gene expression of cervical cancer cell line. Methods: Anti-HPV16E6 ribozyme （ HRz） was designed by computer programs and its activity identified by cleavage experiment in vitro before its transfection via lipofectin into CaSKi cells with the empty eucaryotic expression plasmid transfection of the cells also performed

the resultant cells designated as CaSKi-R

CaSKi-P respectively. The morphology and the soft agar forming ability were studied in CaSKi cells and the transfected cells

and the expression of E6

proliferating cell nuclear antigen （ PCNA） and C-erbB-2 genes assayed by flow cytometry. The tumorgenicity of each cell line was evaluated in nude mice receiving inoculations of CaSKi

CaSKi-R and CaSKi-P cells separately

while in one group

both CaSKi and CaS-Ki-R cells were inoculated on different sides of the mice. Results: HRz was able to cleave H

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