Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells

李洪涛; 潘晓明; 樊民; 吴宗贵; 陆惠琦; 屠小卿; 高春芳

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Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells

Updated：2026-03-12

- Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells
- Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells
- 解放军医学杂志（英文版） 2004年第6期页码：350-352
- Affiliations：
  
  1. Department of Cardiovasology
  2. Changzheng Hospital
  3. Second Military Medical University
  4. ,China
  5. Department of Laboratory Diagnosis
- Author bio：
- Funds：
- DOI：
  中图分类号： R543
- 纸质出版：2004
- Accepted：
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Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells[J]. 解放军医学杂志（英文版）, 2004,(6):350-352.

[1]李洪涛,潘晓明,樊民,吴宗贵,陆惠琦,屠小卿,高春芳.Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells[J].Journal of Medical Colleges of PLA,2004(06):350-352+356.
Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells[J]. 解放军医学杂志（英文版）, 2004,(6):350-352. DOI：

[1]李洪涛,潘晓明,樊民,吴宗贵,陆惠琦,屠小卿,高春芳.Induction of cyclooxygenase-2 by insulin in cultured rat vascular smooth muscle cells[J].Journal of Medical Colleges of PLA,2004(06):350-352+356. DOI：

摘要

Abstract

<正>Objective: To evaluate cyclooxygenase-2 （COX-2） expression induced by insulin in cultured rat vascular muscle smooth cells （VSMCs）. Methods: The rat VSMCs were isolated and cultured in 60 mm plates

pre-treated with insulin 10

100

500

1 000 mU/L for 6 h

and 100 mU/L for 3

and 24 h

respectively. RT-PCR was performed to measure COX-2 mRNA induction

and Western-blot was used to measure protein expression. In regard to the COX-2 enzyme activity

after stimulation for a specified duration

the culture medium was collected; Prostaglandin E2 （PGE2） released by VSMCs was measured with an EIA kit. Results: Three hours after insulin （100 mU/L） stimulation

the induction of COX-2 mRNA was detectable and increased with the time and dosage of insulin. Insulin induced COX-2 protein expression occurred in a time- and dose-dependent manner. In rat VSMCs

COX-2 induced by insulin resulted in PGE2 production

which was increased with insulin action. Conclusion: Insulin could induce COX-2 expression and PGE2

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