Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats

蒋登金; 郭光金; 张坤; 兰阳军; 王林; 张天飞; 左艳芳

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Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats

Updated：2026-03-12

- Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats
- Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats
- 解放军医学杂志（英文版） 2004年第5期页码：267-271
- Affiliations：
  
  1. Department of Applied Surgical Anatomy and Operative Surgery Third Military Medical University
  2. ,China
- Author bio：
- Funds：
  
  Supported by the Foundation for Sci & Tech Research Project of Chongqing, China （2003）
- DOI：
  中图分类号： R392
- 纸质出版：2004
- Accepted：
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Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J]. 解放军医学杂志（英文版）, 2004,(5):267-271.

[1]蒋登金,郭光金,张坤,兰阳军,王林,张天飞,左艳芳.Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J].Journal of Medical Colleges of PLA,2004(05):267-271.
Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J]. 解放军医学杂志（英文版）, 2004,(5):267-271. DOI：

[1]蒋登金,郭光金,张坤,兰阳军,王林,张天飞,左艳芳.Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J].Journal of Medical Colleges of PLA,2004(05):267-271. DOI：

摘要

Abstract

<正>Objective: To estimate directly phagocytic functions of the macrophages because of the importance in innate immunity

determine blood vessel density and re-innervation density which are basis of function. Methods: Eighty adult Wistar rats were randonjy divided into experimental and control groups.The formers underwent splenotomy and a half splenie slice was transplanted into greater omentum. The latter only moved. After 6 months

examination was made as follows: ① After injection of 0.4% carbon particles by vein

spleme tissues were taken out at different times for estimating phagocytosis by light microscope. ② When splenic tissues had been intubated into left ventricle under total anesthesia

animals were perfused by formalin and India ink mixture suspension. Splenic tissues were taken out for making sections for measurement of area density of blood vessels.③ Immunohistochemical procedure was used for detecting neuropeptide Y（NPY）. Results: Phagocytie functions had no difference between two groups

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