Expression and characterization of Mac-1-FP fusion protein in CHO cells

刁飞; 严鸣; 朱晓燕; 杨勇骥; 刘辉; 徐仁宝

Your Location：

Home >

Browse articles >

Expression and characterization of Mac-1-FP fusion protein in CHO cells

Updated：2026-03-12

- Expression and characterization of Mac-1-FP fusion protein in CHO cells
- Expression and characterization of Mac-1-FP fusion protein in CHO cells
- 解放军医学杂志（英文版） 2004年第6期页码：321-324
- Affiliations：
  
  1. Department of Pathophysiology
  2. College of Basic Medical Sciences
  3. Second Military Medical University
  4. ,200438
  5. Department of Cell Biology
  6. International Cooperation Laboratory on Signal Transduction
  7. Eastern Hepatobiliary Surgery Institute
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China （No.30000068, 39730210）
- DOI：
  中图分类号： Q26
- 纸质出版：2004
- Accepted：
Scan QR Code
Expression and characterization of Mac-1-FP fusion protein in CHO cells[J]. 解放军医学杂志（英文版）, 2004,(6):321-324.

[1]刁飞,严鸣,朱晓燕,杨勇骥,刘辉,徐仁宝.Expression and characterization of Mac-1-FP fusion protein in CHO cells[J].Journal of Medical Colleges of PLA,2004(06):321-324.
Expression and characterization of Mac-1-FP fusion protein in CHO cells[J]. 解放军医学杂志（英文版）, 2004,(6):321-324. DOI：

[1]刁飞,严鸣,朱晓燕,杨勇骥,刘辉,徐仁宝.Expression and characterization of Mac-1-FP fusion protein in CHO cells[J].Journal of Medical Colleges of PLA,2004(06):321-324. DOI：

摘要

Abstract

<正>Objective: To construct mammalian cell expression vectors for Mac-1 with CFP and YFP and apply FRET to study the dimerization and function of CD11 b（ Mac-1 α subunit） and CD18（Mac-1 β subunit）. Methods: The mammalian cell expression vector for CD11b fused with CFP at the carboxyl terminal was constructed to create recombinant plasmid of pCD11b-CFP. Then pCD11b-CFP was co-transfected with pYFP-CD18 into CHO cell

a fibroblast like cell line

as a target cell within which there are some signal pathways involved in inflammatory stimulation but without endogenous Mac-1. Then CHO cells stably expressing both CD11b-CFP and YFP-CD18 fusion proteins were selected by Western blot and laser scanning confocal microscope. Results: The cyan and yellow fluorescence in co-transfected positive CHO cells were observed under a fluorescence microscope. CHO-Mac-1-FP cells stably expressing both CD11b-CFP and YFP-CD18 fusion proteins were obtained as demonstrated by Western blot successfully. The adhesive activity of CHO-M

关键词

Keywords

references

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据