<正>Objective: To investigate the effects of motilin and erythromycin on intracellular Ca2+ mobi-lization in cultured myenteric neurons of rats. Methods: The cultured myenteric neurons were identified with immunofluorescence staining technique. Motilin-induced and erythromycin-induced intracellular Ca2+ mobilization was studied in primary cultures of myenteric neurons using the ratiometric Ca2+ indicator Furo3/AM

with a laser confocal microscope. Results: The effects of motilin and erythromycin on intracellular Ca2+ mobilization were as follows: （1）In Hank’s solution

10 -8

10-7

10-6 mol/L motilin could elevate intracellular Ca2+ concentration （[Ca2+]i）in a dose-dependent manner. （2） In Hank’s solution

10μg/ ml erythromycin also could induce the elevation of [Ca2+]i. （3） After pretreatment with antibody against the motilin receptor in Hank’s solution

the Ca2+ response to erythromycin was almost restricted. Conclusion: It is suggested that motilin could increase [Ca2+]i in myenteric neurons in a dose-dependent manner

and erythromycin may also have this effectivenesss by binding to the motilin receptor.