<正>Objective: To establish the rat model with myocardial hypoxia/reoxygenation （H/R） injury

and investigate the protective effect of EPO pretreatment on the myocardium. Methods: Sixty male adult Wistar rats were randomly divided into 3 groups: control group

H/R group

and EPO group

20 in each group. The rats in EPO group accepted injection of 5 000 U/kg recombinant human erythropoietin （RHuEPO） through vein

and the other rats accepted the injection of the same volume of saline. Twenty-four hours after the injection

rats in the EPO and H/R groups were put into the hypoxia environment for 12 h and then returned to the normoxic environment for 2 h

and then the samples of blood and myocardium were collected. Serum myocardial enzyme activity

apoptosis

ultrastructure

myocardial MDA contents

EPO receptor （EPOR） expression in cardiac myocytes and cardiac functions were tested. Results: EPOR expression was positive in cardiac myocytes of adult rat according to the result of immunonistochemitry assaying. Co