Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats

张岫竹; 熊健琼; 刘媛; 曾琳; 龙在云; 张良; 周继红; 朱佩芳; 王正国

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Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats

Updated：2026-03-12

- Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats
- Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats
- 解放军医学杂志（英文版） 2006年第1期页码：30-33
- Affiliations：
  
  1. Fourth DepartmentState Key Laboratory of Trauma Burns and Combined Injuries
  2. Research Institute of Surgery
  3. Daping Hospital
  4. Third Military Medical University
  5. ,China
- Author bio：
- Funds：
- DOI：
  中图分类号： R346
- 纸质出版：2006
- Accepted：
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Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats[J]. 解放军医学杂志（英文版）, 2006,(1):30-33.

[1]张岫竹,熊健琼,刘媛,曾琳,龙在云,张良,周继红,朱佩芳,王正国.Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats[J].Journal of Medical Colleges of PLA,2006(01):30-33.
Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats[J]. 解放军医学杂志（英文版）, 2006,(1):30-33. DOI：

[1]张岫竹,熊健琼,刘媛,曾琳,龙在云,张良,周继红,朱佩芳,王正国.Effects of high dose glucocorticoid on expression and mRNA transcription of corticotropin releasing hormone in hypothalamus paraventricular nucleus of rats[J].Journal of Medical Colleges of PLA,2006(01):30-33. DOI：

摘要

Abstract

<正>Objective: To explore the effect of high dose of glucocorticoid (GO on the synthesis of corticotropin releasing hormone （CRH） and transcription of its mRNA in hypothalamus paraventricular nuclei （PVN） in order to investigate its difference with that of traditional GC effects and to add a new possible explanation to the mechanism of clinical applications of high dose of GC. Methods: A total of 60 rats were divided into 5 groups; blank control. 10’’ mol/L dexamethasone （DEX） group. 10-9 mol/L DEX group

0. 9% saline group and GR blocking group （102mol/L RU486）. All agents were administrated through the femoral vein. CRH protein expression was measured by immunohistochemistry and laser confocal scanning microscopy （LCSM）; CRH mRNA level was explored by in situ hybridization. Results: 10-6 mol/L DEX made CRH mRNA transcripted after 20 min and its protein expressed in PVN after 30 min

while normal level of DEX and 0. 9% saline could not. If GR was blocked in advance

the effect of high dose of DEX disappeared. Conclusion; High dose of GC can have CRH increased in PVN

which differs to the effect of traditional GC. And mGRH may play an important role in the effect of high dose of GC but not classic iGR.

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